                         SEQUENCE LISTING

<110>  IPSEN MANUFACTURING IRELAND LIMITED
       DONG, Zheng Xin
       LOUGHMAN, Thomas C
       HURLEY, Fionn
       JOHNSON, Steven R
 
<120>  Process for the Synthesis of (Aib8,35)hGLP-1(7-36)-NH2

<130>  162P/PCT2

<150>  US 61/192,939
<151>  2008-09-22

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  1

His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg 
            20                  25                  30  


<210>  2
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic analogue of glucagon-like peptide-1 (GLP-1)


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa = alpha-aminoisobutyric acid (Aib)

<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  Xaa = alpha-aminoisobutyric acid (Aib)

<220>
<221>  MOD_RES
<222>  (30)..(30)
<223>  AMIDATION

<400>  2

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Xaa Arg 
            20                  25                  30  


<210>  3
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic analogue of glucagon-like peptide-1 (GLP-1)


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa = alpha-aminoisobutyric acid (Aib)

<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  Xaa = alpha-aminoisobutyric acid (Aib)

<220>
<221>  MOD_RES
<222>  (30)..(30)
<223>  modified with resin

<400>  3

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Xaa Arg 
            20                  25                  30  


<210>  4
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic analogue of glucagon-like peptide-1 (GLP-1)


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa = alpha-aminoisobutyric acid (Aib)

<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa = alpha-aminoisobutyric acid (Aib)

<220>
<221>  MOD_RES
<222>  (29)..(29)
<223>  modified with resin

<400>  4

Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln 
1               5                   10                  15      


Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Xaa Arg 
            20                  25                  


<210>  5
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic analogue of glucagon-like peptide-1 (GLP-1)


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  modified with tert-butyloxycarbonyl (Boc) protecting group at 
       N-terminus

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa = alpha-aminoisobutyric acid (Aib)

<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  Xaa = alpha-aminoisobutyric acid (Aib)

<220>
<221>  MOD_RES
<222>  (30)..(30)
<223>  modified with resin

<400>  5

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Xaa Arg 
            20                  25                  30  


<210>  6
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic analogue of glucagon-like peptide-1 (GLP-1)


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa = alpha-aminoisobutyric acid (Aib)

<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  modified with tert-butyl ester (OtBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  modified with tert-butyl ether (tBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (6)..(6)
<223>  modified with tert-butyl ether (tBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  modified with tert-butyl ether (tBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  modified with tert-butyl ester (OtBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  modified with tert-butyl ether (tBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  modified with tert-butyl ether (tBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  modified with tert-butyl ether (tBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  modified with tert-butyl ester (OtBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  modified with trityl (Trt) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (19)..(19)
<223>  modified with tert-butyloxycarbonyl (Boc) sidechain protecting 
       group

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  modified with tert-butyl ester (OtBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (24)..(24)
<223>  modified with tert-butyloxycarbonyl (Boc) sidechain protecting 
       group

<220>
<221>  MOD_RES
<222>  (27)..(27)
<223>  modified with tert-butyloxycarbonyl (Boc) sidechain protecting 
       group

<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa = alpha-aminoisobutyric acid (Aib)

<220>
<221>  MOD_RES
<222>  (29)..(29)
<223>  2,2,4,6,7-pentamethyldihydrobenzofuran-5-sulfonyl (Pbf) sidechain
       protecting group

<220>
<221>  MOD_RES
<222>  (29)..(29)
<223>  modified with resin

<400>  6

Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln 
1               5                   10                  15      


Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Xaa Arg 
            20                  25                  


<210>  7
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic analogue of glucagon-like peptide-1 (GLP-1)


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  modified with tert-butyloxycarbonyl (Boc) protecting group at 
       N-terminus

<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  modified with trityl (Trt) sidechain protecting group

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa = alpha-aminoisobutyric acid (Aib)

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  modified with tert-butyl ester (OtBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  modified with tert-butyl ether (tBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  modified with tert-butyl ether (tBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  modified with tert-butyl ether (tBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  modified with tert-butyl ester (OtBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  modified with tert-butyl ether (tBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  modified with tert-butyl ether (tBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (13)..(13)
<223>  modified with tert-butyl ether (tBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  modified with tert-butyl ester (OtBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  modified with trityl (Trt) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  modified with tert-butyloxycarbonyl (Boc) protecting group

<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  modified with tert-butyl ester (OtBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (25)..(25)
<223>  modified with tert-butyloxycarbonyl (Boc) protecting group

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  modified with tert-butyloxycarbonyl (Boc) sidechain protecting 
       group

<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  Xaa = alpha-aminoisobutyric acid (Aib)

<220>
<221>  MOD_RES
<222>  (30)..(30)
<223>  modified with 2,2,4,6,7-pentamethyldihydrobenzofuran-5-sulfonyl 
       (Pbf) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (30)..(30)
<223>  modified with resin

<400>  7

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Xaa Arg 
            20                  25                  30  


<210>  8
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic analogue of glucagon-like peptide-1 (GLP-1)


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa = alpha-aminoisobutyric acid (Aib)

<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa = alpha-aminoisobutyric acid (Aib)

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  AMIDATION

<400>  8

Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln 
1               5                   10                  15      


Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Xaa 
            20                  25              


<210>  9
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic analogue of glucagon-like peptide-1 (GLP-1)


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa = alpha-aminoisobutyric acid (Aib)

<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  Xaa = alpha-aminoisobutyric acid (Aib)

<220>
<221>  MOD_RES
<222>  (29)..(29)
<223>  AMIDATION

<400>  9

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Xaa 
            20                  25                  


<210>  10
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic analogue of glucagon-like peptide-1 (GLP-1)


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  modified with trityl (Trt) sidechain protecting group

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa = alpha-aminoisobutyric acid (Aib)

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  modified with tert-butyl ester (OtBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  modified with tert-butyl ether (tBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  modified with tert-butyl ether (tBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  modified with tert-butyl ether (tBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  modified with tert-butyl ester (OtBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  modified with tert-butyl ether (tBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  modified with tert-butyl ether (tBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (13)..(13)
<223>  modified with tert-butyl ether (tBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  modified with tert-butyl ester (OtBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  modified with trityl (Trt) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  modified with tert-butyloxycarbonyl (Boc) sidechain protecting 
       group

<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  modified with tert-butyl ester (OtBu) sidechain protecting group

<220>
<221>  MOD_RES
<222>  (25)..(25)
<223>  modified with tert-butyloxycarbonyl (Boc) sidechain protecting 
       group

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  modified with tert-butyloxycarbonyl (Boc) sidechain protecting 
       group

<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  Xaa = alpha-aminoisobutyric acid (Aib)

<220>
<221>  MOD_RES
<222>  (30)..(30)
<223>  2,2,4,6,7-pentamethyldihydrobenzofuran-5-sulfonyl (Pbf) sidechain
       protecting group

<220>
<221>  MOD_RES
<222>  (30)..(30)
<223>  modified with resin

<400>  10

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Xaa Arg 
            20                  25                  30  


